Sudan Black B Certified CAS: 4197-25-5 | MF: C29H24N6 | MW: 456.54, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
Sudan Black B Certified (CAS 4197-25-5) is a high-performance azo dye, which has a deep-black colour and excels in lipid staining. Having a C29H24N6 molecular formula and molecular weight of 456.54 g/mol, the certified dye is commonly used in histology, microscopy and biochemical research laboratories. It is especially useful in the visualization of lipids and proteins because of its high staining intensity, chemical stability and sensitivity to detecting proteins. The Sudan Black B is still one of the surest and most commonly used reagents to identify lipids in biological samples.
Applications
- Used in the Brain BLAQ (Block Lipids and Aldehyde Quench) technique of lipid visualization.
- Good for the staining of phospholipids and lipid deposits within tissues.
- Very sensitive and specific lipid stain; gives a blue-black stain to neutral fats and a gray to phospholipids.
Key features
- Excellent Lipid Affinity: Gives clear and sharp staining on lipid-rich structures to enhance better microscopic analysis.
- High Sensitivity & Precision: Assures proper determination of lipid components in the histological and cytological samples.
- Dependable and stable Composition: Remains chemically intact and yields similar results with repeated use in the laboratory.
- Broad Laboratory Utility: Histology, cytology, hematology, pathology, and advanced biochemical studies were suitable.
General Description
Sudan Black B Certified is a high-quality histological stain that is used to give excellent results in visualizing lipid structures in biological samples. Its differentiation between blue-black on neutral fats and gray on phospholipids is beneficial to increased clarity and contrast in microscopic research. Being a certified-grade lab reagent, it is a reproducible and reliable reagent in both hand-based staining procedures and automated diagnostic tools. It is common in research institutions, and teaching institutions.
To those who seek Sudan Black B Certified suppliers, prices, or bulk buying, Sudan Black B Certified from Chemical World lab-grade dye comes in various packaging options to suit the various research and institutional requirements. Attractive pricing, bulk buying advantages, and timely shipping facilities can guarantee a cost-effective procurement process for all big or small laboratories. To receive information on pricing and bulk supply or tailor-made laboratory needs, please call our support team now. Invest in laboratory reagents, which not only give stable performance but also economic value in the long term. Order Sudan Black B Certified and obtain accuracy in each practical.
About Brand
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding, which allows for the resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under the TriStains series is validated for accuracy, reliability, and consistency. TriStains, which manufactures and markets stains and indicators in various packaging, offers laboratories turnkey solutions for all their staining and indicator needs, improving accuracy in every experiment.
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| Alternate Names | Sudan Black B is also known as Solvent Black 3, Fat Black HB, and CI 26150 |
| Application | Sudan Black B is a fat-soluble diazo dye which has been used to stain phospholipids and intracellular lipids |
| CAS Number | 4197-25-5 |
| Purity | 99% |
| Molecular Weight | 456.54 |
| Molecular Formula | C29H24N6 |
Sudan Black B is primarily used for staining of neutral triglycerides and lipids. Also a histochemical stain for chromosomes, Golgi apparatus, and leucocyte granules. Sudan Black B has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and it stains blue-black. Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. Sudan Black B is not as lipid-specific as some of the other Sudan dyes and may be useful to stain other materials. Sudan Black B is useful for detecting fats that are contaminated with oil and grease. Sudan black B is used in the study of haematological disorders as it will stain myeloblasts, but not lymphoblasts. Sudan Black B can also stain materials ionically; in this case some background staining is to be expected, and interpretation of sections stained with it must take this into account.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol (10 mg/mL), acetone, benzene, toluene, and hydrocarbon. Insoluble in water. |
| Storage | Store at 4° C |
| Melting Point | 120-124° C (lit.) |
| pK Values : | pKb: 10.88 |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | SD4431500 |
| PubChem CID | 61336 |
| Merck Index | 14:8885 |
| MDL Number | MFCD00006919 |
| EC Number | 224-087-1 |
| Beilstein Registry | 723248 |
| SMILES | CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)N= NC6=CC=CC=C6)C=CC=C3N1)C |
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