Rhodamine B CAS: 81-88-9 | MF: C28H31N2O3€š¬¢Cl | MW: 479.01S10168, Part Number S10168, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Rhodamine B is a useful fluorochrome for histology due to its fluorescent chemical properties in ultraviolet light. It ejects an electron onto TiO2, that's then scavenged through O2 forming the O2- radical anion. This anion in the end turns into the OH radical. A pH-impartial process degrades Rhodamine B in TiO2 dispersions containing no dodecylbenzenesulfonate. The compound has been included in a FRET system based on lanthanide-doped nanoparticles for tracking biological consequences. Additionally , Rhodamine B has been used as a mitochondrial probe to study the active enegetic of multi-drug resistant and sensitive cells where the mitochondrial Rhodamine B concentration become precisely determined with the lack of fluorescence in the presence of MTT Formazan (sc-215399), a fluorescence quencher. Further, Rhodamine B has been observed to noticeably reduce the number of human lip fibroblast cells in culture. Used for staining Negri bodies in tissue at Dawnscientific.com.
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| Alternate Names | Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine |
| Application | A useful fluorochrome for histology, FRET and mitochondrial probe |
| CAS Number | 81-88-9 |
| Molecular Weight | 479.01 |
| Molecular Formula | C28H31N2O3•Cl |
| Supplemental Information | This is classified as a Dangerous Good for transport and may be subject to additional shipping charges |
Rhodamine B is a useful fluorochrome for histology due to its fluorescent properties in ultraviolet light. It ejects an electron onto TiO2, which is then scavenged by O2 forming the O2- radical anion. This anion ultimately becomes the OH radical. A pH-independent process degrades Rhodamine B in TiO2 dispersions containing no dodecylbenzenesulfonate. The compound has been incorporated in a FRET system based on lanthanide-doped nanoparticles for monitoring biological effects. Rhodamine B has also been used as a mitochondrial probe to study the energetic state of multi-drug resistant and sensitive cells where the mitochondrial Rhodamine B concentration was precisely determined with the loss of fluorescence in the presence of MTT Formazan (sc-215399), a fluorescence quencher. In addition, Rhodamine B has been observed to significantly reduce the number of human lip fibroblast cells in culture. Used for staining Negri bodies in tissue.
| Usage | Emission recorded at 545 nm |
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water (12 mg/ml), ethanol (70 mg/ml), acetone (slightly ), methyl cellosolve and alcohol. |
| Storage | Store at room temperature |
| Melting Point | 210-211° C (lit.)(dec.) |
| Density | 1.31 g/cm3 |
| Fluoresence | ?ex 554 nm, ?em 627 nm in ethanol |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | BP3675000 |
| PubChem CID | 6694 |
| Merck Index | 14: 8183 |
| MDL Number | MFCD00011931 |
| EC Number | 201-383-9 |
| Beilstein Registry | 4119648 |
| SMILES | [Cl-].CCN(CC)C1=CC=C2C(OC3=CC(C=CC3=C2C2=C (C=CC=C2)C(O)=O)=[N+](CC)CC)=C1 |
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