Ponceau S Electrophoresis CAS: 6226-79-5 | MF: C22H12N4Na4O13S4 | MW: 760.57S10154, Part Number S10154, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Ponceau S Electrophoresis belongs to the azo dye family and is recognized for its vibrant red hue. The molecular formula of Ponceau S Electrophoresis is C22H12N4Na4O13S4. Also referred to as Ponceau S or Ponceau Red, its chemical name is 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt. This synthetic dye is commonly utilized in protein staining, especially in electrophoresis.
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations.
TriStains chromatic performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.
Application :
- Ponceau S Electrophoresis is widely used to stain proteins separated by gel electrophoresis techniques, such as SDS-PAGE and Western blotting.
- It is commonly used to stain protein bands on nitrocellulose or PVDF membranes after protein transfer.
- The intensity of the Ponceau S Electrophoresis staining can be used to estimate the relative abundance of proteins in a sample, providing a simple and cost-effective method for protein quantification.
Benefits :
- Gives reliable results
- Versatile and compatible
- Cost-effective and easy to use
| Alternate Names | Ponceau S |
| Application | Ponceau S solution is a rapid ready to use protein band stain for western blots and proteins on cellulose acetate membranes |
| CAS Number | 6226-79-5 |
| Molecular Weight | 760.57 |
| Molecular Formula: | C22H12N4Na4O13S4 |
Ponceau S stain comes ready-to-use and is designed for rapid (5 minutes) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1 M NaOH for 1 min. The Ponceau S stained and destained membrane can then be immunologically detected (Western blotting). Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. Ponceau S solution also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.)
| Usage | Ponceau S Staining Solution contains 0.1% Ponceau S (w/v) and 5.0% acetic Acid (w/v). The product is at working concentration, to be used as sold without further dilution. |
| Formulation | 0.1 % (w/v) in 5% acetic acid |
| Physical State | Liquid |
| Solubility | Soluble in water (1 mg/ml). Insoluble in ether. |
| Storage | Store at room temperature |
| Refractive Index | n20D 1.34 |
SAFETY INFORMATION
| WGK Germany | 1 |
| PubChem CID | 6364532 |
| MDL Number | MFCD00003892 |
| EC Number | 228-319-2 |
| SMILES | C1=CC(=CC=C1N=NC2=CC(=C(C=C2)NN=C3C4=C (C=C(C=C4)S(=O)(=O)[O-])C=C(C3=O)S(=O)(=O)[O-]) S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+] |
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