Ponceau S Electrophoresis CAS: 6226-79-5 | MF: C22H12N4Na4O13S4 | MW: 760.57, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
Ponceau S Electrophoresis is an artificial azo dye that is bright red in colour. Its molecular formula is C22H12N4Na4O13S4 and it is usually known as Ponceau S or Ponceau Red. It is chemically known as the 3-Hydroxy-4(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt. The dye is mostly employed in staining of proteins, specifically in electrophoresis procedures.
Applications
- Protein Electrophoresis: Widely used to stain proteins separated by gel electrophoresis methods.
- Membrane Staining: Commonly applied to visualize protein bands on nitrocellulose or PVDF membranes after protein transfer.
Key features
- Reliable Results: The staining of protein bands is uniform, well-defined, and easy to observe.
- Versatile and Compatible: Applicable to the widely used membranes like nitrocellulose and PVDF.
- Low Cost and easy to use: Simple staining procedure that provides a reproducible outcome in standard laboratory procedures.
General Description
Ponceau S Electrophoresis is a molecular weight 760.57 protein electrophoresis azo dye used widely in the protein electrophoresis procedure and is a laboratory grade. The dye gives a clear red stain that enables the scientist to determine the efficiency of protein transfer, check the integrity of the bands, and ensure its suitability to further analytical treatments. Its rapid staining of protein, its resistance to chemical breakdown, and compatibility with standard membrane materials are reasons why it is useful to molecular biology, histology and protein analysis laboratories.
Want to buy Ponceau S Electrophoresis? Chemical World offers high-purity Ponceau S Electrophoresis dye that is applicable in a professional laboratory setting where the performance and quality are paramount. It is tested on a batch-by-batch basis in order to guarantee reproducibility and reliable testing results. The size of packaging, order quantities, requirements of purity, and customization affect the prices. The sales department can be approached by the customers to get updated pricing, technical documentation, and bulk order discounts. Buy Ponceau S Electrophoresis from the Chemical World because it provides reliable, precise, and repeatable staining results.
About Brand
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding, which allows for the resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under the TriStains series is validated for accuracy, reliability, and consistency. TriStains, which manufactures and markets stains and indicators in various packaging, offers laboratories turnkey solutions for all their staining and indicator needs, improving accuracy in every experiment.
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| Alternate Names | Ponceau S |
| Application | Ponceau S solution is a rapid ready to use protein band stain for western blots and proteins on cellulose acetate membranes |
| CAS Number | 6226-79-5 |
| Molecular Weight | 760.57 |
| Molecular Formula: | C22H12N4Na4O13S4 |
Ponceau S stain comes ready-to-use and is designed for rapid (5 minutes) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1 M NaOH for 1 min. The Ponceau S stained and destained membrane can then be immunologically detected (Western blotting). Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. Ponceau S solution also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.)
| Usage | Ponceau S Staining Solution contains 0.1% Ponceau S (w/v) and 5.0% acetic Acid (w/v). The product is at working concentration, to be used as sold without further dilution. |
| Formulation | 0.1 % (w/v) in 5% acetic acid |
| Physical State | Liquid |
| Solubility | Soluble in water (1 mg/ml). Insoluble in ether. |
| Storage | Store at room temperature |
| Refractive Index | n20D 1.34 |
SAFETY INFORMATION
| WGK Germany | 1 |
| PubChem CID | 6364532 |
| MDL Number | MFCD00003892 |
| EC Number | 228-319-2 |
| SMILES | C1=CC(=CC=C1N=NC2=CC(=C(C=C2)NN=C3C4=C (C=C(C=C4)S(=O)(=O)[O-])C=C(C3=O)S(=O)(=O)[O-]) S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+] |
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