Fluorescein Diacetate

Fluorescein Diacetate CAS: 596-09-8 | MF: C24H16O7 | MW: 416.38, Buy now for reliable and reproducible results from Tristains at Chemical World.

Fluorescein Diacetate (FDA) is an unfluorescent form of fluorescein having the molecular formula C24H16O7. It is also known as 3,6- Diacetoxyfluoran or Di-O-acetylfluorescein, and it easily enters cell membranes. It is broken down by esterases to produce fluorescein and a bright green fluorescence is formed in viable cells. This process renders Fluorescein Diacetate an effective, selective labelling and quantification agent of living cells in microbiology and cell biology.

Applications

• Cell Biology and Live-Cell Imaging: Fluorescein Diacetate is widely used in cell viability assays. While non-fluorescent outside the cell, it is enzymatically converted to fluorescent fluorescein within viable cells, allowing clear differentiation between live and non-viable cells.
• Bacterial Viability Testing and Microbiology: Applied in the determination of metabolic and esterase activity of bacterial cells, which supplements microbial viability investigations.
• Flow Cytometry and Automated High-Throughput Screening: Works with fluorescent-based flow cytometry systems and automated platforms of large-scale analysis.

Key features

• High Sensitivity: Detects very low levels of esterase activity, enabling precise cell viability determination.
• Broad Range of Applications: Appropriate to microorganisms, fungi, plant cells, and mammalian cells.
• Stable: Gives repeatable and reliable results in repeated experiments and with long shelf life.
• Economical: Economical in large-scale research and recurrent assays.

General Description

Fluorescein Diacetate (CAS No. 596-09-8) is a common fluorescent viability reagent that is applicable in biological and diagnostic laboratories. It is an excellent selective marker of cellular activity because of its non-fluorescent nature in the absence of cells and its enzymatic activation in viable cells. Its analytical performance is great and its molecular weight is 416.38 g/mol, which enables the sensitive detection of enzymatic processes and tissue viability in research and quality control contexts.

Want to buy Fluorescein Diacetate? Chemical World offers fluorescent dyes and stains of laboratory grade to research, academic, and industrial laboratories. Fluorescein Diacetate is packaged in different sizes in order to meet the laboratory requirements. The costs are based on the amount ordered and the type of packaging and the prices are offered at a discount. Contact Chemical World to enquire about the existing prices or order.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.