Brilliant Blue G250 | CAS: 6104-58-1 | MF: C47H48N3O7S2·Na | MW: 854.02 | Buy now for reliable and Reproducible results from TriStains at Chemical World.
Brilliant Blue G250 is a dark blue to purple synthetic stain that is commonly used to stain proteins in a biochemical and molecular biology application. This dye is also referred to as Coomassie Brilliant Blue G-250 (C.I. 42655) and it is a triphenylmethane dye family. It has a C47H48N3NaO7S2 molecular formula. Brilliant Blue G250 is also known as Acid blue 90, Brilliant blue, Cyanine G, Polar blue, SERVA BLUE G, and protein gel stain. The dye is selective towards proteins and can be easily visualized at low concentrations in a sensitive manner.
Applications
- Brilliant Blue G250 finds wide application in protein visualization and separation in sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) during which proteins are presented as blue spots on a clear background.
- Applied in Bradford protein assay which is commonly used to determine protein amount.
- The migration patterns of proteins in gels stained with Brilliant Blue G250 help in the estimation of the size of a protein.
Key features
- Very sensitive protein detection.
- Solid and rapid attachment to proteins.
- Consistent and accurate staining.
- Very good solubility and storage stability.
General Description
Brilliant Blue G250 (CAS No. 6104-58-1) is a protein stain that has been in use in research labs. Having a molecular weight of 854.02 and Color Index number C.I. 42655, this dye offers stable functionality in protein electrophoresis, assays and analysis processes.
Chemical World offers Brilliant Blue G250 to research laboratories, biotechnology plants as well as academic institutions with guaranteed quality and technical documentation. Prices are determined by the size of packaging and grade of purity. The customer can call customer care to know the current prices, bulk supply packages, and shipping information. Select Brilliant Blue G250 from Chemical World due to its reliability in protein staining and analytical quality.
About Brand
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding, which allows for the resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under the TriStains series is validated for accuracy, reliability, and consistency. TriStains, which manufactures and markets stains and indicators in various packaging, offers laboratories turnkey solutions for all their staining and indicator needs, improving accuracy in every experiment.
Chemical World provides the best laboratory brands that provide a constant and reliable result for any kind of experiment. Our range of high-quality laboratory brands is complete and is available in various scientific fields. ChemieR supplies high-purity chemicals to be used in reliable laboratories, cUSP supplies certified and standard solutions which can be used right away to assess accurately, and TriStains provides high-quality histological stains and indicators to be used in order to visualize accurately. LiChrom is the provider of liquid chromatography using high-grade reagents, and Bluster is the provider of high-grade solvents used in gas chromatography. EKS focuses on electronics-grade reagents that are used in advanced and sensitive analysis. These brands assure the research, analytical, and industrial laboratories with trusted quality, consistency, and performance. To get your product details, specifications, or to seek any purchasing assistance, visit our site chemicalworlds.com or email our sales team sales@chemicalworlds.com. We are happy to service your laboratory requirements with professional and fast service.
| Alternate Names | Coomassie Brilliant Blue G (CBBG) |
| Application | Brilliant Blue G is a commonly used analytical biochemistry dye and P2X7 antagonist |
| CAS Number | 6104-58-1 |
| Molecular Weight | 854.02 |
| Molecular Formula: | C47H48N3O7S2†â„ƒ¬…¡¬ÂNa |
Brilliant Blue G (CBBG) is a member of the Coomassie dye family and is commonly used in analytical biochemistry to stain proteins in SDS-PAGE and BN-PAGE. CBBG is a useful component in the Bradford Method, a dye-binding protein assay employed to determine protein concentration in a given solution. CBBG can exist in three forms: a cation (red form with maximum absorbance at 470 nm), a neutral form (green with maximum at 650 nm), and an anionic form (blue with maximum at 595 nm). The anionic form initiates a stable complex with basic amino acid residues, mainly arginine and aromatic amino acids. Although the anionic form exists in negligible concentrations at the assay reaction mixture's pH, the protein-binding shifts the equilibrium toward its formation. Recent research reveals CBBG as a P2X7 (P2X purinoceptor 7: P2RX7) antagonist and suggests that it exhibits neuroprotective properties in rat spinal cord cells.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water (40 mg/ml at 20,° C), methanol, ethanol (40 mg/ml), DMSO (~10 mg/ml), and DMF (~0.5 mg/ml). |
| Storage | Store at room temperature |
| Melting Point | >100,° C |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | 6324599 |
| MDL Number | MFCD00078482 |
| EC Number | 228-058-4 |
| Beilstein Registry | 5230822 |
| SMILES | CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC(=C(C=C2)/C(=C/3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3C)/C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C.[Na+] |
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