Trypan Blue CAS: 72-57-1 | MF: C34H24N6O14S44Na | MW: 960.81, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
Trypan Blue is a black inorganic powder that has a wide range of applications in research and laboratory work in biology. It is also known as Direct blue 14 or Niagara blue 3B and its molecular formula is C34H24N6O14S4Na4. Trypan Blue is a crucial stain that is commonly used to selectively stain the dead cells so that scientists can easily determine the viability of the cells. It is a reliable reagent in histology, cell biology and microbiology due to its reproducibility, ease of use and chemical stability.
Applications
- Applied in cell culture laboratories to distinguish between dead cells and live cells to enable proper viability measurements.
- Is used as a critical tissue and cellular stain to be examined under the microscope.
- Used in microbiology to determine the viability of bacteria and fungi.
Key features
- Selective Cell Staining: Stains dead cells while leaving viable cells unstained, ensuring accurate experimental outcomes.
- Consistent and Reliable: Gives repeatable figures in biological, histological, and microbiological research.
- Flexible and Cost-Effective: Applicable to a variety of staining processes, it is compatible with cell culture, microbiology and histology applications.
General Description
Trypan Blue is a common vital dye commonly utilized in cell viability studies, tissue staining and microbiological tests (CAS: 72-57-1). Its selective staining capabilities, solubility and chemical stability render it one of the most important tools in research, education, and laboratory practice.
Want to buy Trypan Blue? Chemical World provides high-purity Trypan Blue that provides reliable and reproducible outcomes in cell biology, microbiology, and histology studies in the USA. Packaging and price differ depending on the amount and quantity; inquire with the supplier on bulk pricing and present rates on laboratory-grade Trypan Blue. Get the latest pricing, quantity breaks, and quotes by calling the supplier or going online. Every batch is manufactured to ensure consistency in quality, purity, and reliable performance. Order Trypan Blue today from Chemical World for reliable and reproducible laboratory results.
About Brand
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding, which allows for the resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under the TriStains series is validated for accuracy, reliability, and consistency. TriStains, which manufactures and markets stains and indicators in various packaging, offers laboratories turnkey solutions for all their staining and indicator needs, improving accuracy in every experiment.
Chemical World provides the best laboratory brands that provide a constant and reliable result for any kind of experiment. Our range of high-quality laboratory brands is complete and is available in various scientific fields. ChemieR supplies high-purity chemicals to be used in reliable laboratories, cUSP supplies certified and standard solutions which can be used right away to assess accurately, and TriStains provides high-quality histological stains and indicators to be used in order to visualize accurately. LiChrom is the provider of liquid chromatography using high-grade reagents, and Bluster is the provider of high-grade solvents used in gas chromatography. EKS focuses on electronics-grade reagents that are used in advanced and sensitive analysis. These brands assure the research, analytical, and industrial laboratories with trusted quality, consistency, and performance. To get your product details, specifications, or to seek any purchasing assistance, visit our site chemicalworlds.com or email our sales team sales@chemicalworlds.com. We are happy to service your laboratory requirements with professional and fast service.
| Alternate Names | Direct Blue 14, Dianil blue, Diaminine Blue |
| Application | Trypan Blue is a viability dye for quantifying live versus dead cells |
| CAS Number | 72-57-1 |
| Molecular Weight | 960.81 |
| Molecular Formula: | C34H24N6O14S44Na |
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water (10 mg/ml at 25° C), ethanol (0.6 mg/ml), and EGME (20 mg/ml). |
| Storage | Store at 4° C |
| Melting Point | >300° C (lit.) |
| IC50 | SH-PTP2: IC50 = 3.9 µM (human); Protein-tyrosine phosphatase 1: IC50 = 7.4 µM (Saccharomyces cerevisiae); P2Y2: IC50 = 17.1 µM (human) |
| pK Values | pKa: -1.53 (Predicted), pKb: 2.39 (Predicted) |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | QJ6475000 |
| PubChem CID | 9562061 |
| Merck Index | 14: 9792 |
| MDL Number | MFCD00003969 |
| EC Number | 200-786-7 |
| Beilstein Registry | 4360496 |
| SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC (=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-])C)NN=C5C (=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-]) S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+] |
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