Trypan Blue CAS: 72-57-1 | MF: C34H24N6O14S4€š¬¢4Na | MW: 960.81S10193, Part Number S10193, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Titan Yellow, also known as Direct Yellow 11 or Solvent Yellow 44, is a synthetic dye belonging to the class of azo dyes. It is commonly used in various industrial applications, including textiles, plastics, and paper at Dawnscientific.com.
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| Alternate Names | Direct Blue 14, Dianil blue, Diaminine Blue |
| Application | Trypan Blue is a viability dye for quantifying live versus dead cells |
| CAS Number | 72-57-1 |
| Molecular Weight | 960.81 |
| Molecular Formula: | C34H24N6O14S4•4Na |
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water (10 mg/ml at 25° C), ethanol (0.6 mg/ml), and EGME (20 mg/ml). |
| Storage | Store at 4° C |
| Melting Point | >300° C (lit.) |
| IC50 | SH-PTP2: IC50 = 3.9 µM (human); Protein-tyrosine phosphatase 1: IC50 = 7.4 µM (Saccharomyces cerevisiae); P2Y2: IC50 = 17.1 µM (human) |
| pK Values | pKa: -1.53 (Predicted), pKb: 2.39 (Predicted) |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | QJ6475000 |
| PubChem CID | 9562061 |
| Merck Index | 14: 9792 |
| MDL Number | MFCD00003969 |
| EC Number | 200-786-7 |
| Beilstein Registry | 4360496 |
| SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC (=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-])C)NN=C5C (=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-]) S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+] |
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