Sudan Black B Certified CAS: 4197-25-5 | MF: C29H24N6 | MW: 456.54S10181, Part Number S10181, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Sudan Black B is a synthetic dye that has been used historically in the laboratory setting for various applications at Dawnscientific.com.
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| Alternate Names | Sudan Black B is also known as Solvent Black 3, Fat Black HB, and CI 26150 |
| Application | Sudan Black B is a fat-soluble diazo dye which has been used to stain phospholipids and intracellular lipids |
| CAS Number | 4197-25-5 |
| Purity | 99% |
| Molecular Weight | 456.54 |
| Molecular Formula | C29H24N6 |
Sudan Black B is primarily used for staining of neutral triglycerides and lipids. Also a histochemical stain for chromosomes, Golgi apparatus, and leucocyte granules. Sudan Black B has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and it stains blue-black. Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. Sudan Black B is not as lipid-specific as some of the other Sudan dyes and may be useful to stain other materials. Sudan Black B is useful for detecting fats that are contaminated with oil and grease. Sudan black B is used in the study of haematological disorders as it will stain myeloblasts, but not lymphoblasts. Sudan Black B can also stain materials ionically; in this case some background staining is to be expected, and interpretation of sections stained with it must take this into account.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol (10 mg/mL), acetone, benzene, toluene, and hydrocarbon. Insoluble in water. |
| Storage | Store at 4° C |
| Melting Point | 120-124° C (lit.) |
| pK Values : | pKb: 10.88 |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | SD4431500 |
| PubChem CID | 61336 |
| Merck Index | 14:8885 |
| MDL Number | MFCD00006919 |
| EC Number | 224-087-1 |
| Beilstein Registry | 723248 |
| SMILES | CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)N= NC6=CC=CC=C6)C=CC=C3N1)C |
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