Pyronin Y Certified CAS: 92-32-0 | MF: C17H19N2OCl | MW: 302.8, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
Pyronin Y Certified (CAS No. 92-32-0) or Pyronin G or Pyronin J: a molecular formula of C17H19N2O.Cl and a molecular weight of 302.8. It is highly known as one of the reliable stains in histology and is generally applied in the ethyl (methyl) greenpyronine method of identifying nucleic acid as an exact reagent. Also, Pyronin Y Certified is a fluorescent stain, which exhibits high fluorescence when stained on DNA and RNA in biological research.
Applications
- Pyronin Y is actively used in histology and is included in the methyl greenpyronine technique of staining RNA and DNA.
- Pyronin Y can be employed as a differentiable fluorescent stain to detect and visualize nucleic acids when it is utilized with Methyl Green.
Key features
- High Specificity and Sensitivity: Pyronin Y Certified (CAS: 92-32-0) offers great specificity and high sensitivity in the process of staining nucleic acid which is very reliable and reproducible in laboratory studies.
- Versatility: It is applicable in various research and diagnostic settings as this dye is applicable to a broad spectrum of tissue preparations and staining procedures.
- Quality and Certification: Certified Pyronin Y guarantees adherence to the quality, reliability, and performance, which is why it is perfect to use in the case of critical research and analytical processes.
General Description
Pyronin Y Certified (CAS: 92-32-0) is a commonly used fluorescent nucleic acid stain used in laboratory research for the visualization of RNA or DNA. It has a molecular formula of C17H19N2O2Cl and a molecular weight of 302.8, which makes it an inevitable laboratory reagent in the histology, cytology, microbiology, hemology and other related fields of biological science.
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About Brand
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding, which allows for the resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under the TriStains series is validated for accuracy, reliability, and consistency. TriStains, which manufactures and markets stains and indicators in various packaging, offers laboratories turnkey solutions for all their staining and indicator needs, improving accuracy in every experiment.
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| Alternate Names | Pyronin G, Pyronin J, Pyronin Y |
| Application | Pyronin Y is a dye that intercalates and stains RNA red |
| CAS Number | 92-32-0 |
| Molecular Weight | 302.80 |
| Molecular Formula: | C17H19N2OÂCl |
Pyronin Y is a dye that intercalates and stains RNA red, and has been shown to accumulate in the mitochondria of viable cells. Multidrug-resistant (MDR) Acinetobacter baumannii studies tested inhibition of the efflux pump by measuring the intracellluar accumulation of Pyronin Y and Ethidium bromide (sc-203947). Research shows that at low Pyronin Y concentrations the cytostatic effect is cell arrest in the G1 phase, whereas at high concentrations the cells arrest is in the G2 and S phase. Pyronin Y has been used in combination with Alcian Blue (sc-214517) to asses and study pulmonary diseases, and in combination with Methyl Green (sc-215348) to stain nucleic acids in paraffin sections. Additionally Pyronin Y has been used to measure RNA in flow cytometry.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water (30 mg/mL), methyl cellosolve (9 mg/mL), and ethanol (3 mg/mL). |
| Storage | Store at room temperature |
| Melting Point | 250-260° C (lit.) |
| Density | 0.64 g/cm3 at 20 |
| IC50 | HEK293 (Embryonic kidney fibroblasts): IC50 = 4540 nM (human) |
| Fluoresence | ex 546 nm, em 565 nm in DMSO |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | BQ1450000 |
| PubChem CID | 7085 |
| Merck Index | 14: 8007 |
| MDL Number | MFCD00011725 |
| EC Number | 202-147-8 |
| Beilstein Registry | 3795789 |
| SMILES | CN(C)C1=CC2=C(C=C1)C=C3C=CC(=[N+](C)C)C=C3O2.[Cl-] |
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