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Nile Red

Nile Red CAS: 7385-67-3 | MF: C20H18N2O2 | MW: 318.37S10134, Part Number S10134, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.

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Nile Red, also known as Nile Blue Oxazone, is a greenish red crystalline powder with the chemical formula C20H18N2O2. It is a lipophilic stain that is used to stain intracellular lipid droplets yellow. This fluorescent dye has various applications, especially in the fields of biological and analytical chemistry. Its chemical name is 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations.

TriStains chromatic performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

  • Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells.
  • It has applications in cell biology, where it can be used as a membrane dye which can be readily visualized using an epifluorescence microscope with excitation and emission wavelengths usually shared with red fluorescent protein.

Benefits :

  • Exhibits a strong fluorescence signal when in a hydrophobic environment
  • Versatile and cost-effective
Alternate Names Nile Red is also known as 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one and 9-(diethylamino)benzo[a]phenoxazin-5-one.
Application Nile Red is a photostable, lipophilic stain with bright red fluorescence that has been used stain cell structures to visualize and localize colloidal dru
CAS Number 7385-67-3
Molecular Weight 318.37
Molecular Formula: C20H18N2O2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. * Refer to Certificate of Analysis for lot specific data (including water content).Nile Red is an uncharged, hydrophobic, photostable molecule that strongly fluoresces bright red in hydrophobic environments. Nile Red is a lipophilic stain commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile Red has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. Nile Red has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Nile Red s fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites.
Appearance Powder
Physical State Solid
Solubility Soluble in water (Partly miscible), and methanol (1 mg/ml).
Storage Store at room temperature
Melting Point 203-205° C (lit.)
Boiling Point 484.70° C at 760 mmHg (Predicted)
Density ~1.2 g/cm3 (Predicted)
Refractive Index n20D 1.64 (Predicted)

SAFETY INFORMATION

WGK Germany 3
PubChem CID 65182
Merck Index 14: 10229
MDL Number MFCD00011639
EC Number 230-966-0
Beilstein Registry 279110
SMILES CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2

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