Neutral Red, 90% CAS: 553-24-2 | MF: C15H17ClN4 | MW: 288.78, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
Neutral Red 90% (CAS No. 553-24-2) is a synthetic crystallized powder, which is black-brown to dark green in solid form, and gives a red solution in water. It has a molecular formula of C15H17ClN4 and is categorized as a water-soluble dye. Neutral Red 90% is very commonly used in staining and labeling processes and it serves as an indicator in the preparation of neutral red paper and also as a biological stain. Neutral Red is frequently used in histology and cytology to stain tissue sections and cellular smears, and is used as a pH indicator with a color change between red and yellow occurring in the pH range 6.8 to 8.0. It has numerous applications in biology as a biological stain, an acid-base indicator, and a two-color indicator, and has the cationic form of neutral red (1+).
Applications
- Cell Viability Testing: Neutral Red 90% is common in cell viability assays, in which it diffuses passively through cellular membranes and is specifically taken up into viable cells, allowing assessment of cell health and activity.
- Cell Nuclear Stain and Cytoplasm Stain: Neutral Red is used as a nuclear stain, counterstain, as well as in Gram staining techniques as a Gram counterstain. Being a red cationic fluorochrome, it can be used in staining cells and in a multi-contrast bacterial classification.
- Routine Laboratory Use and Teaching: In learning laboratories, Neutral Red is usually employed to showcase live cell staining in a range of cell cultures. It is an acid-base indicator, with a low toxicity and safety profile, and thus it can be used in routine laboratory and teaching use.
Key features
- Proper Viability Indicator: Stains only viable cells, making it possible to accurately measure the health and metabolic activity of cells.
- Multi-Functional: Uses as a biological dye, pH indicator, and acid-base two-color indicator.
- Easy to Use: Dissolves in water, enabling easy application to a variety of staining uses.
- Versatile Applications: Suited to histological, cytological and fluorescent staining methods.
- Cost-Effective: Neutral Red 90% provides a dependable performance as well as a cost-effective price to laboratories of any size.
General Description
Neutral Red 90% (CAS No. 553-24-2) is an artificial dye with wide applications in biological studies and microscopy. It is known to be a strong staining agent and to be used as an acid-base indicator in histology, cytology, and cell viability studies. Having a molecular formula of C15H17ClN4 and a molecular weight of 288.78, this dye is vital in labs that are concerned with tissue and cell visualization in microscopy. Neutral Red 90% can be used in histology, cytology, microbiology, hematology, and biology laboratories.
Want to buy Neutral Red 90%? Chemical World offers certified-grade histology stains and dyes that are used to satisfy the strict demands of laboratory research, diagnosis, and routine settings. Competitive shipping service, easy-to-use packaging choices and attentive customer service make sure that Neutral Red 90% is easily accessible in the academic research, diagnostic pathology, and industrial biology applications. Neutral Red 90% pricing depends on quantity, packaging choice and custom specification. There are competitive prices and the chance of making bulk purchases without losing the quality and performance of the product. In live-cell analysis, tissue staining and indicator-based assays, Neutral Red 90% is a vital lab stain that has offered precision, consistency and reproducibility when purchased from Chemical World. Purchase Neutral Red 90% Solution from Chemical World for dependable, high-performance staining across laboratory applications.
About Brand
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding, which allows for the resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under the TriStains series is validated for accuracy, reliability, and consistency. TriStains, which manufactures and markets stains and indicators in various packaging, offers laboratories turnkey solutions for all their staining and indicator needs, improving accuracy in every experiment.
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| Alternate Names | 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; Basic Red 5; Toluylene red |
| Application | Neutral Red is a common histology stain used to determine cell viability. |
| CAS Number | 553-24-2 |
| Molecular Weight | 288.78 |
| Molecular Formula | C15H17ClN4 |
Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evalutaion of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm.
| Appearance | Powder |
| Physical State | Solid |
| Solubility |
Soluble in water (50 mg/ml at 25° C), abs. alcohol (1.8%), and cellosolve (3.75%).
|
| Storage | Store at 4° C |
| Melting Point | 290° C (lit.)(dec.) |
| Boiling Point | 497.3° C at 760 mmHg |
| Refractive Index | n20D 1.75 (Predicted) |
| IC50 : | Internal ribosome entry site (IRES): IC50= 20 µM (human); Huh-7: IC50= 69.26 µM (human); -Arrestin-1: IC50= 8.88 µM (human); Cytoplasmic zinc-finger protein: IC50= 13.00 µM (Caenorhabditis elegans); Apaf-1: IC50= 34 µM (human) |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | SG1400000 |
| PubChem CID | 11105 |
| Merck Index | 14:6488 |
| MDL Number | MFCD00012651 |
| EC Number | 209-035-8 |
| Beilstein Registry | 3918740 |
| SMILES | CC1=CC2=NC3=C(C=C(C=C3)N(C)C)N=C2C=C1N.Cl |
Safety Data Sheets (SDS), Product Specification (PS) and Certificates of Analysis (COA) click Document Button