Methylene Blue Certified CAS: 7220-79-3 | MF: C16H18N3S€š¬¢Cl€š¬¢3H2O | MW: 373.9S10117, Part Number S10117, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Methylene blue is a compound that presents itself as either crystals or crystalline powder, with a shine similar to bronze. Its chemical formula is C16H24ClN3O3S. It can dissolve in water and chloroform, and is partially soluble in alcohol. It is also referred to as Basic Blue 9, Tetramethylthionine chloride. This photoactive dye belongs to the phenothiazine family and is commonly utilized in histology, bacteriology (staining), compounding of blood stains, and has a wide range of applications in various industries.
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations.
TriStains chromatic performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.
Application :
- Methylene blue certified has been used to stain cells, and to evaluate cell survival by staining.
- It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light.
Benefits :
- Ensures consistent purity, potency, and compliance with industry standards
- Versatile and stable
| Alternate Names | C.I. Basic Blue 9, trihydrate (8CI); Methylthionine chloride |
| Application | Methylene blue trihydrate is an inhibitor of cGMP-mediated processes and guanylyl cyclase, a useful stain, and a redox indicator |
| CAS Number | 7220-79-3 |
| Molecular Weight | 373.90 |
| Molecular Formula: | C16H18N3S•Cl•3H2O |
Methylene Blue acts as a GCS (guanylyl cyclase) inhibitor, biological stain and redox indicator. Methylene Blue inhibits tau filament formation. Additionally, Methylene Blue is also reported to inhibit NOSs (nitric oxide synthase). Methylene Blue is widely used to inhibit cGMP-mediated processes. Methylene Blue has been observed to dose-dependently induce cytotoxicity in U-373 MG human astrocytoma and SK-N-MC human neuroblastoma cells. Furthermore Methylene Blue is reported to bind to the M2 subtype of muscarinic receptors in rat cardiac myocytes, and thus exhibit antimuscarinic effects on IK, ACh and ICa. Methylene Blue has also been reported to inhibit endothelium-dependent relaxation of isolated blood vessels.
| Appearance | Crystalline powder |
| Physical State | Solid |
| Solubility | Soluble in water (50 mg/ml), ethanol (70 mg/ml), alcohol, and chloroform. Insoluble in ether. |
| Storage | Store at room temperature |
| Melting Point | 190° C |
| IC50 : | Plasmodium falciparum: IC50 = 5.4 nM; Glutathione reductase: IC50 = 6.4 µM (Plasmodium falciparum 3D7); Apaf-1: IC50 = 15.5 µM (human); Glutathione reductase: IC50 = 16 µM (human) |
| pK Values : | pKb: 4.89 (Predicted) |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | SP5740000 |
| PubChem CID | 104827 |
| Merck Index | 14:6060 |
| MDL Number | MFCD00150008 |
| EC Number | 200-515-2 |
| Beilstein Registry | 3599847 |
| SMILES : | CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.O.O.[Cl-] |
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