Giemsa Stain Certified CAS: 51811-82-6 | MF: C14H14ClN3S | MW: 291.8S10095, Part Number S10095, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Giemsa Stain Certified displays a color ranging from dark green to very dark green. Its chemical formula is C14H14ClN3S and it is also known as Azure mixture sicc. Giemsa stain is a versatile polychromatic stain suitable for staining a wide variety of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. The staining solution is composed of methylene blue, azure, and eosin. It is certified for Giemsa stain for blood films and for the Wolbach?s Giemsa method for paraffin sections. Giemsa Stain Certified is a high-quality stain commonly used in cytogenetics, histology, and parasitology.
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Application :
- Giemsa€š¬€ž¢s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites.
- It is frequently used in combination with other dye solutions as May-Grunwald€š¬€ž¢s solution for Pappenheim (MGG) and Wright-Giemsa.
- The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements.
- Giemsa stain has been used for the staining of cells and blood films. It is required for the staining of azurophil granules and is an excellent stain for inclusion bodies.
- Its applications can be seen in hematology for blood and bone marrow specimens, bacteriology, clinical cytology specimens, histological biopsies, and tumor samples.
Benefits :
- Ensures that the stain maintains a high level of purity and consistency
- Produces consistent and reliable staining results
- Versatile and effective
Giemsa Stain is a laboratory reagent and stain for microscopy. The stain functions by rearranging chromatin and inducing G bands producing a high quality stain for chromatin and the nuclear membrane. Giemsa Stains have been used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain has also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain. However, the May-Grunwald-Giemsa procedure produces a more intense coloration than Wright-Giemsa.
| Alternate Names | Giemsa solution |
| Application | Giemsa Stain is a high quality stain for chromatin and the nuclear membrane |
| CAS Number | 51811-82-6 |
| Molecular Weight | 291.8 |
| Molecular Formula | C14H14ClN3S |
SAFETY INFORMATION
woodmart_empty_space=""| PubChem CID | 13735 |
| MDL Number | MFCD00081642 |
| EC Number | 257-438-2 |
| SMILES | C[N+](=C1C=CC2=NC3=C(C=C(C=C3)N)SC2=C1)C.[Cl-] |
| Usage : | Technique used to uniformly stain chromosomes that will leave centromeres constricted: Method 1 materials: 4 mL Giemsa; 96 mL pH 6.8 phosphate buffer procedure: 1.Place slides in a Coplin jar or staining dish. 2.Prepare the working stain and pour it over the slides. Stain for 7 minutes. 3.Rinse slides in two changes of distilled water. 4.Air dry slides; mount them with a cover slip if desired. (If sequential banding procedures are to follow, cover slipping is not recommended.) Method 2 materials: 1.5N HCl 2.Distilled water 3.pH 6.8 phosphate buffer 4. Giemsa stain: 2 mL stock Giemsa; 4 mL pH 6.8 buffer; 92 mL distilled water procedure: 1.Place slides in a Coplin jar containing 5N HCl for 10 minutes at room temperature. 2.Rinse with tap water for 10 minutes. 3.Stain for in Giemsa for 10 minutes. 4.Rinse, dry, and coverslip, if desired. |
| Physical State | Solid |
| Solubility | insoluble in water (<0.1%), methanol (5.8 mg/l), and ethanol. |
| Storage | Store at room temperature |
| Melting Point | 300° C (lit.) |
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