Gentian Violet USP CAS: 548-62-9 | MF: C25H30N3€š¬¢Cl | MW: 407.99S10094, Part Number S10094, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Gentian Violet USP, a synthetic dye derived from coal tar, is a triarylmethane dye that can be found in the form of a dark green or violet powder. When dissolved in water or alcohol, it creates a deep violet solution. Its chemical formula is C25H30N3Cl. This dye is also referred to as Crystal Violet, Basic Violet 3, Hexamethylpararosaniline chloride, and Methyl Violet 10B. It is used in laboratory setting. It is commonly utilized in a variety of fields, such as histology, microscopy, and microbiology.
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations.
TriStains chromatic performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.
Application :
- Gentian Violet USP is used in certain histological techniques to stain tissue sections, highlighting cellular structures and aiding in microscopic examination.
- It is used as a primary stain in the Gram staining technique to differentiate Gram-positive bacteria (which retain the violet color) from Gram-negative bacteria.
- It is employed in various staining protocols to visualize and identify microorganisms.
Benefits :
- Effective against a wide range of pathogens, including bacteria and fungi
- Relatively inexpensive and widely available
- Provides clear and distinct staining results
| Alternate Names | Gentian Violet; Hexamethylpararosaniline chloride |
| Application | Crystal Violet is a component of Gram staining in microorganisms and cell lines |
| CAS Number | 548-62-9 |
| Molecular Weight | 407.99 |
| Molecular Formula: | C25H30N3•Cl |
| Supplemental Information: | This is classified as a Dangerous Good for transport and may be subject to additional shipping charges. |
Crystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to assess bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water (50 mg/ml at 27° C), chloroform, alcohol (partially soluble), and glycerol (partially soluble). Insoluble in ether. |
| Storage | Store at room temperature |
| Melting Point | 205° C (lit.)(dec.) |
| Density : | 1.19 g/cm3 at 20° C |
| IC50 : | Sodium Channel, Site 2 radioligand binding: IC50= 460 nM; Protein Tyrosine Kinase, EGF Receptor enzyme inhibition: IC50= 473 nM; Muscarinic M5 radioligand binding: IC50= 535 nM; Alpha-2A adrenergic receptor radioligand binding: IC50= 536 nM |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | LM5850000 |
| PubChem CID | 11048 |
| Merck Index | 14:3603 |
| MDL Number | MFCD00005040 |
| EC Number | 241-409-6 |
| Beilstein Registry | 3586809 |
| SMILES | C1=CC=C(C(=C1)C2=C3C=C(C(=O)C(=C3OC4=C(C(=C(C=C24)Br)[O-])Br)Br)Br)C(=O)[O-].[Na+].[Na+] |
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