Fuchsin Basic Certified CAS: 632-99-5 | MF: C20H19N3€š¬¢HCl | MW: 337.86S10092, Part Number S10092, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Basic fuchsin Certified is a member of the triphenylmethane group. It is a synthetic dye commonly utilized in biological and microbiological staining. This substance presents itself as a dark green crystalline powder that forms a magenta solution once dissolved in water or alcohol. Its chemical formula is C19H17N3 · HCl. Additionally, it is recognized by various names such as Basic Parafuchsin, Basic Red 9, Magenta‚¬ž¢ O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, and Pararosaniline hydrochloride. It is certified for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff?s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques. Basic fuchsin is predominantly employed for staining bacteria, chromosomes, and other cellular structures. This high-quality dye is a reliable choice for microbiological, histological, and cytological staining purposes.
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Application :
- As a primary stain, Fuchsin Basic is used in some variations of the Gram staining procedure to differentiate Gram-positive bacteria (which retain the dye) from Gram-negative bacteria.
- Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes
- Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff?s reagent. This reagent is responsible for the staining of chromosomal DNA.
- Basic fuchsin is also used for the staining of lignin in plant tissue samples.
- It is used in various protocols to stain bacteria, making it easier to observe and identify bacterial morphology and structure under a microscope.
Benefits :
- High quality, with consistent purity and performance
- Provides clear and distinct staining results
- Suitable for a wide range of staining protocols in microbiology, histology, and cytology
Alternate Names | Basic Red 9; Magenta O |
Application | Basic Fuchsin is a flourescent dye used for stainning and for the Ziel-Neelson staining method |
CAS Number | 632-99-5 |
Molecular Weight | 337.86 |
Molecular Formula: | C20H19N3•HCl |
Basic Fuchsin is a dye composed of rosaniline, magenta II, pararosaniline, and new fuschsine. The fluorescent dye has been used for Ziehl-Neelson staining in order to detect acid-fast bacilli. The dyes fluorescence has been useful in understanding microfissures, as it allows a clear delineation of surface level structure and constant background from the microscope. Basic Fuchsin staining includes tissue sections to visualize elastic fibers, and cardiac and skeletal muscle tissue. Reports show that Basic Fuchsin has the ability to stain tubercle Bacillus, bacterial flagella, and to compare Escherichia coli and Aerobacter aerogenes.
Appearance | Crystalline powder |
Physical State | Solid |
Solubility | Soluble in alcohol, water (4 g/l) at 25° C, and acids practic. Insoluble in ether. |
Boiling Point | 570.8° C at 760 mmHg (Predicted) |
Storage | Store at room temperature |
Melting Point | 205° C (dec.) |
Density : | 1.00 g/cm3 at 20° C |
Refractive Index: | n20D 1.33 |
SAFETY INFORMATION
WGK Germany | 3 |
RTECS | CX9850000 |
PubChem CID | 12447 |
Merck Index | 14: 5652 |
MDL Number | MFCD00012569 |
EC Number | 211-189-6 |
Beilstein Registry : | 4166684 |
SMILES : | CC1=CC(=C(C2=CC=C(C=C2)N)C3=CC=C(C=C3)N)C=CC1=N.Cl |
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