Evans Blue C.I. 23860 CAS: 314-13-6 | MF: C34H24N6Na4O14S4 | MW: 960.83S10079, Part Number S10079, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Evans Blue, also known as C.I. 23860 and Direct Blue 53, is a synthetic azo dye with the chemical formula C34H24N6Na4O14S4. It is a dark blue, crystalline powder that dissolves in water and other polar solvents. This dye belongs to the class of diazo dyes and is renowned for its intense, vivid blue color. Evans Blue has a strong affinity for serum albumin, making it an effective high molecular weight protein tracer. This property allows it to be utilized in physiology for estimating the proportion of body water contained in blood plasma. The versatile and valuable nature of Evans Blue has led to its widespread applications in biomedical research, analytical chemistry, and various industrial sectors, taking advantage of its distinctive properties and color characteristics.
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Application :
- In histology, Evans Blue is used as a staining agent, particularly in combination with other dyes, to enhance the visualization of tissues, cells, and cellular structures under a microscope.
- In the field of biomedical research, it is widely used as a tracer dye to study vascular permeability and blood-tissue barrier integrity.
- Evans Blue is employed in various protein binding assays, such as albumin-binding studies, due to its ability to bind to serum albumin.
Benefits :
- Ensures the longevity of the color in various applications
- Versatile and stable
- Valuable colorant in various applications where a bold, vibrant blue is desired
| Alternate Names | Direct blue 53; CI 23860 |
| Application | Evans Blue is an inhibitor of EAAT-mediated L-glutamate uptake |
| CAS Number | 314-13-6 |
| Molecular Weight | 960.83 |
| Molecular Formula: | C34H24N6Na4O14S4 |
Evans blue is a potent inhibitor of L-glutamate uptake into synaptic vesicles via EAAT. Evans Blue also inhibits AMPA and kainate receptor-mediated currents (IC50 values are 220 and 150 nM respectively). The compound is also a P2X-selective purinoceptor antagonist. Used as a dye, Evans blue reduces unwanted fluorescence due to the conjugation and auto fluorescence of structural elements in the tissue or cells.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water. |
| Ki Data | Vesicular glutamate transporter 3: Ki= 40 nM (rat); Autotaxin: Ki= 824 nM (human) |
| Storage | Store at room temperature |
| pK Values | n20D ~1.84 (Predicted) |
| IC50 : | Autotaxin: IC50 = 922 nM (human); Protein-tyrosine phosphatase 1: IC50 = 1200 nM (Saccharomyces cerevisiae); Protein-tyrosine phosphatase 1 B: IC50 = 1300 nM (human) |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | QJ6440000 |
| PubChem CID | 9566057 |
| Merck Index | 14: 3905 |
| MDL Number | MFCD00004021 |
| EC Number | 206-242-5 |
| Beilstein Registry : | 162259 |
| SMILES : | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N/N=C3/C(=O)C4=C(C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])C=C3)C)N/N=C5/C(=O)C6=C(C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])C=C5.[Na+].[Na+].[Na+].[Na+] |
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