Brilliant Blue G250 CAS: 6104-58-1 | MF: C47H48N3O7S2€š¬¢Na | MW: 854.02, Part Number S10037, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.
.Brilliant Blue G250 typically appears as a dark blue or purple powder. Brilliant Blue G250, also known as Coomassie Brilliant Blue G-250, is a synthetic dye commonly used in biochemistry and molecular biology for protein staining. Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. Its molecular formula is C47H48N3NaO7S2. Brilliant Blue G250 is also known as Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain. The dye has a high affinity for proteins, allowing it to selectively bind to and stain proteins in solution.
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations.
TriStains chromatic performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.
Application :
- Brilliant Blue G250 is widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It is commonly used in the Coomassie Blue staining method, where proteins are visualized as blue bands against a clear background.
- CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.
- The migration pattern of stained proteins in a gel can be used to estimate the size of proteins, as smaller proteins generally migrate faster than larger ones.
Benefits :
- High sensitivity in detecting proteins
- Gives accurate and reliable results
- Cost-effective
| Alternate Names | Coomassie Brilliant Blue G (CBBG) |
| Application | Brilliant Blue G is a commonly used analytical biochemistry dye and P2X7 antagonist |
| CAS Number | 6104-58-1 |
| Molecular Weight | 854.02 |
| Molecular Formula: | C47H48N3O7S2•Na |
Brilliant Blue G (CBBG) is a member of the Coomassie dye family and is commonly used in analytical biochemistry to stain proteins in SDS-PAGE and BN-PAGE. CBBG is a useful component in the Bradford Method, a dye-binding protein assay employed to determine protein concentration in a given solution. CBBG can exist in three forms: a cation (red form with maximum absorbance at 470 nm), a neutral form (green with maximum at 650 nm), and an anionic form (blue with maximum at 595 nm). The anionic form initiates a stable complex with basic amino acid residues, mainly arginine and aromatic amino acids. Although the anionic form exists in negligible concentrations at the assay reaction mixture's pH, the protein-binding shifts the equilibrium toward its formation. Recent research reveals CBBG as a P2X7 (P2X purinoceptor 7: P2RX7) antagonist and suggests that it exhibits neuroprotective properties in rat spinal cord cells.
| Appearance | Powder |
| Physical State | Solid |
| Solubility | Soluble in water (40 mg/ml at 20° C), methanol, ethanol (40 mg/ml), DMSO (~10 mg/ml), and DMF (~0.5 mg/ml). |
| Storage | Store at room temperature |
| Melting Point | >100° C |
SAFETY INFORMATION
| WGK Germany | 3 |
| RTECS | 6324599 |
| MDL Number | MFCD00078482 |
| EC Number | 228-058-4 |
| Beilstein Registry | 5230822 |
| SMILES | CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC(=C(C=C2)/C(=C/3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3C)/C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C.[Na+] |
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