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Acridine Orange

Acridine Orange CAS: 10127-02-3 | MF: C17H19ClN3€š¬¢HCl€š¬¢1/2ZnCl2 | MW: 369.96, Part Number S10007, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Chemical World.

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Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. When acridine orange binds to DNA, the dye exhibits a maximum excitation at 502 nm producing a maximum emission of 525 nm. When bound to RNA, acridine orange displays a maximum emission value of 650 nm and a maximum excitation value of 460 nm. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. The other names of it are 3,6-Acridinediamine, Acridine Orange Base, Acridine Orange NO, Basic Orange 14 etc. Acridine Orange is commonly used as a fluorescent stain in microbiology and histology.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations.

TriStains chromatic performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

  • Acridine orange is useful for cell-cycle studies.
  • It is used as a fluorescent staining agent to detect the presence of bacteria in blood cultures and other bodily fluids.
  • It is useful in the rapid screening of ordinarily sterile specimens.

Benefits :

  • Able to withstand low pH environments
  • Differentiate various types of cells (i.e., bacterial cells and white blood cells)
  • High quality and good effectiveness
Alternate Names 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt)
Application Acridine Orange hemi(zinc chloride) salt is fluorescent dye that stains DNA and RNA
CAS Number 10127-02-3
Molecular Weight 369.96
Molecular Formula
C17H19ClN3•HCl•1/2ZnCl2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. Refer to Certificate of Analysis for lot specific data (including water content).Acridine Orange hemi (zinc chloride) salt is a cell-permeable metachromatic fluorescent dye that stains DNA and RNA. This dye is also an inhibitor of RNA polymerase. References 1. Wolfe, A.D. et al. 1972. Biochemistry. 11(9): 1569-1572. PMID: 4554949 2. Golden, J.F. and West, S.S. 1974. J. Histochem. Cytochem. 22(7): 495-505. PMID: 4136741 3. Golden, J.F. et al. 1979. J. Histochem. Cytochem. 27(1): 522-528. PMID: 374619 4. Frost, J.K. et al. 1979. J. Histochem. Cytochem. 27(1): 545-551. PMID: 86575 5. Tyrer, H.W. et al. 1979. J. Histochem. Cytochem. 27(1): 552-556. PMID: 86576 6. Paul, R.N. 1980. Stain Technol. 55(3): 195-196. PMID: 6161450
Appearance Powder
Physical State Solid
Solubility Soluble in water (1 mg/ml).
Storage Store at room temperature
Melting Point 165° C
Refractive Index
n20D ~1.71 (Predicted)
Fluoresence ?ex 502 nm, ?em 525 nm; ?ex 460 nm, ?em 650 nm

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany 3
RTECS AR7600000
PubChem CID 159263
MDL Number MFCD00081043
EC Number 233-353-6
Beilstein Registry 3734978
SMILES CN(C)C1=CC2=C(C=C1)C= C3C=CC(=CC3=N2)N(C)C.Cl.[Cl-].[Cl-].[Zn+2]

Safety Data Sheets (SDS), Product Specification (PS) and Certificates of Analysis (COA) click Document Button